Figure 5From: A system of vectors for Bacillus subtilis spore surface display Localisation of fusion proteins as assessed by immunofluorescence microscopy. Purified, free spores of wild type strain 168, BAV05 (CgeA-CagA), BAV07 (CgeA-linker-CagA) were visualised by phase contrast (PC) and immunofluorescence (IF) microscopy. The spores were incubated with mouse anti-CagA, followed by anti-mouse IgG-Cy3 conjugates. The same exposure time was used for all IF images. Black and white scale bars – 1 μm.Back to article page