Skip to main content

Table 1 Heterologous proteins produced in Lactococcus lactis.

From: Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production

Proteins Gene Origin Location References
Reporter     
Nuc nuc Staphylococcus aureus Cytoplasmic / secreted / anchored [6, 16]
β-lactamase bla Escherichia coli secreted [44]
β-galactosidase β-gal Clostridium acetobutylicum cytoplasmic [45]
lactamase lacL, lacM Leuconostoc mesenteroides cytoplasmic [46]
α-amylase amyS Geobacillus (formerly Bacillus) stearothermophilus secreted [47] [18]
α-amylase amyL Bacillus licheniformis secreted [48]
Chloramphenicol Acetyl Transferase cat-86 Bacillus pumilus cytoplasmic [49]
M6   Streptococcus pyogenes anchored [12]
Green fluorescent protein gfp Aequoria victoria (jellyfish) cytoplasmic [50]
luciferase luxAB Vibrio harveyi cytoplasmic [51]
luciferase Vf lux Vibrio fischeri cytoplasmic [52]
Streptavidin SA Streptomyces avidinii anchored [11]
β-glucuronidase gus Escherichia coli cytoplasmic [53]
Bacterial antigens     
L7/L12 L7/L12 Brucella abortus Cytoplasmic/secreted/anchored [19]
Urease subunit B   Helicobacter pilori secreted [54]
TTFC ttfc Clostridium tetani secreted [55]
Eukaryotic antigen     
GLURP-MSP3 fusion protein   Plasmodium falciparum secreted [56]
Viral antigens     
E7 E7 HPV type-16 cytoplasmic/secreted/anchored [20] [57]
NSP4 NSP4 Bovine coronavirus cytoplasmic [29]
BCV epitope BCV Bovine coronavirus secreted [58]
VP8 subunit of VP4 VP8* rotavirus secreted [59]
Interleukins     
IL-2 IL-2 Mouse secreted [60]
IL-6 IL-6 Mouse secreted [61]
IL-10 IL-10 Mouse secreted [21]
IL-12 IL-12 Mouse Secreted [22]
IFN-ω IFN-ω Ovine secreted [5]
Allergens     
BLG Blg Bovine cytoplasmic/secreted [13, 30, 36]
  Epitope Blg41–60 Bovine secreted  
Virulence factors     
Fibronectin binding protein A fnbpA Staphylococcus aureus anchored [62]
Clumping factor A clfA Staphylococcus aureus anchored [63]
Clumping factor A and B clfB Staphylococcus aureus anchored [64]
serine-aspartate repeat protein sdrE Staphylococcus aureus anchored [64]
Protein A spA Staphylococcus aureus anchored [11]
Enterotoxin A sea Staphylococcus aureus secreted C. Charlier(a)
Unpublished results
Aggregation substance asc10 Enterococcus faecalis anchored [65]
Capsular polysaccharides cps genes Streptococcus pneumoniae CPS excreted [66]
Internalin inlA Listeria monocytogenes anchored V. Guimarães(b)
Unpublished results
Bacteriocins     
ABP-118 abp118 Lactobacillus salivarius subsp. salivarius secreted [67]
Enterocin A ent genes Enterococcus faecium secreted [68]
Pediocin PA-1 ped genes Pediococcus acidilactici secreted [68]
colicin V   Escherichia coli secreted [69]
Enzymes     
heat-stable alpha-glucosidase malA Sulfolobus solfataricus cytoplasmic [70]
Bacteriophage lytic enzyme ply 118 Listeria monocytogenes bacteriophage secreted [71]
lysozyme hel Hen egg white cytoplasmic [72]
Neutral protease npr Bacillus subtilis secreted [73]
Aminopeptidase N pepN Lactobacillus helveticus secreted [74]
Cell Surface Protease prtB Lactobacillus delbrueckii subsp. bulgaricus anchored [13]
Dextrane sucrase dsrD Leuconostoc mesenteroides secreted [28]
Streptodornase sdc Streptococcus equisimilis secreted [75]
prochymosin PC Bovine secreted [76]
lipase lip Staphylococcus hyicus secreted [77]
plasmin   Bovine secreted [78]
others     
F18 fimbrial adhesin (receptor binding domain) fedF Escherichia coli Secreted / anchored [27]
S-layer protein slpH Lactobacillus helveticus cell wall associated [79]
  1. (a) : Laboratoire de Microbiologie UMR1253 INRA Agrocampus, 65 rue de Saint Brieuc CS84215, 35042 Rennes cedex
  2. (b) : Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France