Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production

Table 2 Comparison of the protein yields in secreted vs cytoplasmic production.

Protein	Quantification of the secreted form¹	Quantification of the cytoplasmic form¹	Ratio sec/cyto	References
Nuc	20 mg/L	3 mg/L	6	[5]
L7/L12	3 mg/L	0.5 mg/L	6	[19]
E7 (expo)*	nd	nd	2 to 3	[20]
E7 (stat)*	nd	nd	> 10	[20]
IFN-ω	309 mg/L	159 mg/L	2	[5]

1: protein samples were adjusted to the cell density and protein quantification was performed as described in the references either by western blot or by ELISA.
*: E7 was not quantified but ratio was calculated by scanning the western blot signals and comparing their intensity as described in the corresponding reference.
nd: not determined

ISSN: 1475-2859