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Table 3 Effect of the signal peptide and of the insertion of the LEISSTCDA synthetic propeptide on the secretion efficiency.

From: Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production

Protein SEa with SPNuc SE with SPUsp45 Reference
Nuc 60 % >95 % [25]
NucT 30 % 30 % [25]
Protein SE without LEISS SE with LEISS Reference
Nuc 60 % 80 % [18]
NucT 30 % 90 % [25]
L7/L12 35 % 50 % [19]
AmySb + +++ [18]
  1. a: SE, secretion efficiency is the proportion of total protein which is present in the mature secreted form.
  2. b: SE was not determined by western blot and immuno revelation and thus could not be quantified but the activity plate assay demonstrated a clear secretion enhancement (+ to +++) with LEISS.