Fig. 3From: Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeastsFlow-cytometry analysis of R. toruloides, C. curvatus and L. starkeyi. Overlaid histograms of R. toruloides (a), C. curvatus (b) and L. starkeyi (c) cells grown on pure (left panels) and crude glycerol (right panels) stained with Nile Red and analyzed through flow-cytometry after 28, 48, 72, 144, 192 (only R. toruloides and C. curvatus) and 240 h (only C. curvatus). The fluorescence emission was measured in the FL3 channel (>650 nm corresponding to polar lipids). For each condition, an example of control (cells not stained) is reported. Results shown are representative of three independent experiments, where the deviation from the X mean value was always less than 5 %Back to article page