Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts

Table 1 Comparison of parameters related to biomass and lipid production among R. toruloides, C. curvatus and L. starkeyi grown on pure and crude glycerol

Yeast	Carbon source	Maximum DW (g L⁻¹)	Biomass productivity (g L⁻¹ h⁻¹)^a	Biomass yield (g g⁻¹)	Glycerol uptake rate (g L⁻¹ h⁻¹)^a	Lipid (%)	Lipid productivity (g L⁻¹ h⁻¹)
R. toruloides	Pure glycerol	40.4	0.21	0.33	0.73	60.9	0.13
R. toruloides	Crude glycerol	41.0	0.24	0.36	0.98	60.0	0.15
C. curvatus	Pure glycerol	43.7	0.21	0.38	0.52	46.9	0.10
C. curvatus	Crude glycerol	45.1	0.18	0.37	0.48	50.9	0.09
L. starkeyi	Pure glycerol	31.4	0.31	0.26	1.03	48.2	0.10
L. starkeyi	Crude glycerol	32.7	0.29	0.26	1.05	55.9	0.13

Data shown are the mean of three independent experiments where the deviation from the mean value was less than 5 %
^aBiomass productivity as well as glycerol uptake rate were calculated starting to the end of the feeding

ISSN: 1475-2859