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Table 3 Gas chromatography analysis of R. toruloides, C. curvatus and L. starkeyi lipids produced using crude and pure glycerol as a sole carbon source

From: Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts

Yeast

Carbon source

Fatty acids composition (% wt/wt)

C 14:0

C 16:0

C 16:1

C 18:0

C 18:1

C 18:2

C 18:3

Other

S

M

P

R. toruloides

Pure glycerol

1.5 ± 0.1

27.9 ± 0.1

2.1 ± 0.1

12.3 ± 0.1

35.3 ± 0.1

17.4 ± 0.2

2.8 ± 0.1

0.7 ± 0.1

42.4 ± 0.2

37.4 ± 0.1

20.2 ± 0.2

Crude glycerol

1.5 ± 0.1

27.5 ± 0.1

2.0 ± 0.3

12.5 ± 0.2

37.8 ± 0.1

15.8 ± 0.2

2.2 ± 0.1

0.7 ± 0.1

42.2 ± 0.1

39.8 ± 0.4

18.0 ± 0.3

C. curvatus

Pure glycerol

1.6 ± 0.3

29.6 ± 0.1

3.7 ± 0.3

18.6 ± 0.1

27.6 ± 0.2

14.9 ± 0.1

1.9 ± 0.2

2.1 ± 0.6

51.9 ± 0.1

31.3 ± 0.1

16.8 ± 0.2

Crude glycerol

1.6 ± 0.3

30.6 ± 0.1

4.0 ± 0.3

15.4 ± 0.1

31.2 ± 0.1

14.5 ± 0.1

1.6 ± 0.1

1.1 ± 0.1

48.7 ± 0.4

35.2 ± 0.4

16.1 ± 0.1

L. starkeyi

Pure glycerol

3.1 ± 0.3

31.0 ± 0.1

4.3 ± 0.1

12.9 ± 0.1

39.4 ± 0.3

7.6 ± 0.1

0.5 ± 0.1

1.2 ± 0.3

48.2 ± 0.4

43.7 ± 0.3

8.1 ± 0.1

Crude glycerol

3.0 ± 0.2

32.2 ± 0.1

4.2 ± 0.1

10.9 ± 0.1

40.9 ± 0.1

7.2 ± 0.1

0.6 ± 0.1

1.0 ± 0.2

47.1 ± 0.1

45.1 ± 0.1

7.8 ± 0.2

  1. Peak areas less than 0.3 % were considered insignificant
  2. Data are mean ± standard deviation of three independent assays
  3. S saturated; M monounsaturated; P polyunsaturated