Table 1 Bacterial strains and plasmids used in this study

Strains

 M. neoaurum DSM 1381

Mutant of M. neoaurum ATCC 25790 which can accumulate HPD, 4HP and ADD

[11]

 ΔkstD1

Mutant of M. neoaurum DSM 1381 deleted kstD1

This study

 HK1/HK2/HK3

ΔkstD1 harboring pMV306hsp-kstD1/pMV306hsp-kstD2/pMV306hsp-kstD3

This study

 PK1/PK2/PK3

ΔkstD1 harboring pMV306Pk1-kstD1/pMV306Pk2-kstD2/pMV306Pk3-kstD3

This study

 BL21-pET-28a(+)

E. coli BL21 (DE3) harboring empty plasmid pET-28a(+)

This study

 BL21-kstD1/BL21-kstD2/BL21-kstD3

E. coli BL21 (DE3) harboring plasmid pET28a-kstD1/pET28a-kstD2/pET28a-kstD3

This study

 6051a-pHT01

B. subtilis 6051a harboring empty plasmid pHT01

This study

 6051a-kstD1/6051a-kstD2/6051a-kstD3

B. subtilis 6051a harboring plasmid pHT01-kstD1/pHT01-kstD2/pHT01-kstD3

This study

Plasmids

 PJV53

pLAM12 carrying Che9c 60–61 under control of the acetamidase promoter

[38]

 pGOAL19

Source of hygromycin (Hyg) cassette

[37]

 pET-28a (+)

E. coli expression vector, Kan^R

Novagen

 pHT01

B. subtilis expression vector pHT01; containing Pgrac promoter; Amp^R for E. coli; Cm^R for B. subtilis

[34]

 pMV306

Mycobacterium integrative vector with single copy, without hsp60 promoter, Kan^R

[39]

 pMV306hsp

pMV306 contains hsp60 promoter

[40]

 pET24a-K1UHD

pET-24a(+) contains upstream and downstream of kstD1 gene flanking Hyg cassette

This study

 pHT01-kstD1/pHT01-kstD2/pHT01-kstD3

pHT01 possessing kstD1 or kstD2 or kstD3 gene

This study

 pET28a-kstD1/pET28a-kstD2/pET28a-kstD3

pET-28a (+) possessing kstD1 or kstD2 or kstD3 gene

This study

 pMV306hsp-kstD1/pMV306hsp-kstD2/pMV306hsp-kstD3

pMV306hsp possessing kstD1 or kstD2 or kstD3 gene

This study

 pMV306Pk1- kstD1

pMV306 carrying the 441 bp upstream region together with kstD1 gene

This study

 pMV306Pk2-kstD2

pMV306 carrying the 726 bp upstream region together with kstD2 gene

This study

 pMV306Pk3- kstD3

pMV306 carrying the 1347 bp upstream region together with kstD3 gene

This study