Plasmid | CDWb(g/l) | PHA contentc(wt%) | Significantdifferenced | PHA composition (mol %)e |
---|
3HB | 3HHx | 3HO | 3HD | 3HDD |
---|
pYC2 | 3.23±0.03 | 0.81±0.06 | - | 32.35±1.16 | ND f | 10.61±2.84 | 57.04±3.66 | ND |
pYC2QK | 3.15±0.12 | 1.41±0.06 | 0.019 | 46.00±2.84 | ND | 6.90±0.53 | 47.10±2.82 | ND |
pYC2QKST | 3.13±0.06 | 1.79±0.10 | 0.008 | 41.59±3.43 | ND | 6.28±0.56 | 49.93±2.77 | 2.21±0.09 |
pYC2O | 3.39±0.04 | 3.40±0.05 | 0.004 | 39.49±0.36 | 5.93±0.05 | 6.97±0.06 | 45.33±0.24 | 2.27±0.02 |
pYC2OH | 3.59±0.11 | 3.93±0.11 | 0.004 | 39.59±0.23 | 5.80±0.12 | 6.91±0.12 | 45.46±0.27 | 2.24±0.07 |
- a Cells were cultivated at 37°C and 200 rpm for 48 h as described in “Methods”. 8 g/l dodecanoate were added to the MS medium. Three parallel experiments were conducted for each datum.
- b CDW: cell dry weight.
- c PHA content: PHA contents are given as mass percentage of CDW.
- d Significant difference: p value calculated by student’s t-test, indicating the significant difference between the respective and the previous PHA contents.
- e 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.
- f ND: not detected.