Figure 2
From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
Vectors and strains of the LIKE system. (A) Vector maps of integrative plasmid pLIKE-int and E. coli/B. subtilis shuttle vector pLIKE-rep. Abbreviations: PliaI(opt), liaI promoter with optimized SD sequence; bla, ampicilin resistance; erm, erythromycin resistance; cat, chloramphenicol resistance; ColE1, origin of replication for E. coli; ori1030, origin of replication for B. subtilis. (B) Sequence of the multiple cloning sites for each plasmid. The optimized SD sequence is indicated in bold, the 7 bp ‘spacer’ is boxed, the first nucleotide of the coding sequence is underlined. For pLIKE-int, ClaI can be used as restriction enzyme to create an ATG start codon. For pLIKE-rep, XbaI must be used to reconstruct the ATG start codon. (C) Schematic representation of the liaI operon and genotype of deletion strains constructed in this study. Open reading frames are depicted as solid arrows. Promoters of the genes indicated by thin arrows, terminators by hairpin symbols.