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Table 2 Repartition of enzymatic activities produced by T. xylanilyticus grown on various substrates

From: The hemicellulolytic enzyme arsenal of Thermobacillus xylanilyticus depends on the composition of biomass used for growth

Xylanase (IU/mg)a

Xylosidase (mIU/mg)a

Localization

Extracellular

Cytoplasmic

Membrane associated

Extracellular

Cytoplasmic

Membrane associated

glucose

nd

nd

nd

2.2 ± 0.6

11.4 ± 3.8

nd

xylan

21.4 ± 1.6

4.7 ± 0.1

1.6 ± 0.1

4.7 ± 0.6

62.7 ± 5.0

nd

wheat bran

25.9 ± 3.5

4.9 ± 0.8

0.6 ± 0.0

3.1 ± 0.3

96.6 ± 2.5

nd

wheat straw

5.8 ± 0.3

1.7 ±0.2

0.5 ± 0.0

4.2 ± 0.3

18.9 ± 1.0

nd

Feruloyl-esterase (mIU/mg) a

Acetyl-esterase (mIU/mg) a

Localization

Extracellular

Cytoplasmic

Membrane associated

Extracellular

Cytoplasmic

Membrane associated

glucose

12.0 ± 0.8

27.4 ± 1.0

1.5 ± 0.7

109.6 ± 4.6

174.7 ± 13.3

26.6 ± 1.9

xylan

5.8 ± 0.6

23.9 ± 1.1

23.8 ± 2.9

76.2 ± 8.0

289.5 ± 13.0

nd

wheat bran

15.9 ± 2.2

14.3 ± 1.4

43.09 ± 0.7

64.2 ± 5.8

288.8 ± 35.3

nd

wheat straw

15.9 ± 2.7

146.4 ± 2.8

73.1 ± 4.3

130.0 ± 0.7

93.0 ± 14.5

nd

Arabinofuranosidase (mIU/mg) a

 

Localization

Extracellular

Cytoplasmic

Membrane associated

glucose

6.7 ± 0.3

32.2 ± 2.7

1.0 ± 0.0

xylan

16.0 ± 1.0

230.8 ± 2.2

23.9 ± 3.5

wheat bran

17.5 ± 0.2

399.1 ± 27.5

3.51 ± 0.6

wheat straw

0.6 ± 0.0

64.3 ± 1.2

0.7 ± 0.1

  1. a Activities are given in IU per mg of protein for xylanase and in mIU per mg of protein for arabinofuranosidase, feruloyl, acetyl-esterase and xylosidase activities nd: not detected.