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Figure 1 | Microbial Cell Factories

Figure 1

From: Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans

Figure 1

Metagenomic screening, identification and purification of Lpc53E1. a) Identification of clone 53E1 on 1% tributyrin agar, b) Lipase activity of E. coli clones carrying lpc53E1 gene on plasmid, c) Lipolytic activity of lpc53E1 clone on tributyrin agar plate. d) SDS–PAGE gel showing expression of HIS tagged Lpc53E1 lipase protein (indicated by arrow) in E. coli carrying lpc53E1 gene on pBAD/Myc-HisA; L1 – crude extract of uninduced cells; L2- crude extract of induced cells; and L3 - Ni NTA column purified lipase.

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Microbial Cell Factories

ISSN: 1475-2859

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