Figure 2From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentationsCalibration of measurement signals. (A) Cell dry weight versus scattered light intensities measured in microtiter plates; (B) Cell dry weight versus OD measured in the fermenter bypass; (C) GFP fluorescence in the microtiter plate versus GFP fluorescence in the fermenter of the H. polymorpha fermentation; Operation conditions: MTP (H. polymorpha/E. coli): 200 μL filling volume, at 30°C/32°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, gain: 20) and GFP (ex: 485 nm/em: 520 nm, gain: 10); fermenter (H. polymorpha/E. coli): 1.4 L filling volume, at 30°C/32°C temperature, 800/1000 rpm shaking frequency, 1 vvm specific aeration rate, OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm).Back to article page