Figure 3From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentationsDetermination of the different oxygen transfer rates (OTR) in RAMOS. (A) for the chemical 0.5 mM sulfite system; (B) for the E. coli fermentation in WR medium; (C) for the H. polymorpha fermentation in SYN6-MES medium. Operation conditions: 10 mL, 15 mL and 20 mL filling volume, 250 mL Erlenmeyer shake flask for RAMOS, 250 rpm shaking frequency, 50 mm shaking diameter, at 25°C temperature with the sulfite system and 30°C temperature with the microorganisms. The E. coli cultures were induced at 7.17 h with 0.5 mM IPTG.Back to article page