Figure 6From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentationsComparison of parallel H. polymorpha fermentations in microtiter plate and stirred tank fermenter. Operation conditions: MTP: 200 μL filling volume, at 30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter, scattered light (ex: 620 nm/em: -, gain: 20) and GFP (ex: 485 nm/em: 520 nm, gain: 10); fermenter: 1.4 L filling volume, at 30°C temperature, 800 rpm shaking frequency, 1 vvm specific aeration rate, OD (Absorption: 630 nm) and GFP (ex: 480 nm/em: 530 nm).Back to article page