Figure 4

Effect of different solvents on the solubility of EGFP and MMP-12_CAT inclusion bodies. A fixed amount of EGFP and MMP-12_CAT inclusion bodies was suspended in different solvents containing 2 M urea and solubilized by freeze-thawing method. (A) SDS-PAGE analysis of effect of different solvents on the solubility of EGFP inclusion bodies. (B) SDS-PAGE analysis of effect of different solvents on the solubility of MMP-12_CAT inclusion bodies. PBS, PBS at pH 8 with 2 M urea; NaP, 20 mM sodium phosphate at pH 8 with 2 M urea; KP, 20 mM potassium phosphate at pH 8 with 2 M urea; Tris, 20 mM Tris at pH 8 with 2 M urea; H₂O, deionized water at pH 8.5 with 2 M urea. (C) Protein bands were quantitated by densitometric analysis using ImageJ Software.