Fig. 2From: Influence of global gene regulatory networks on single cell heterogeneity of green fluorescent protein production in Bacillus subtilisThe expression of sfGFP(Sp) in various B. subtilis strains. B. subtilis WT, CodYR214C, CcpAT19S, CodYR214CCcpAT19S harboring amyE::Physpank-sfgfp(Sp) were grown in flasks with LB supplemented with 1.0% glucose and 0.1 mM IPTG under the same growth conditions (37 °C, 220 rpm). Samples were harvested for both fluorescence and OD600 measurement per hour. a Flow cytometric analysis of GFP expression. Dotted lines were placed at 103 Arbitrary Units (AU) to serve as a reference of the fluorescence distributions. b The mean fluorescence intensity of the whole population over time. c The optical density at 600 nm of various strains was measured by spectrophotometry. For WT, CcpAT19S, the exponential phase is 1–4 h, and start the stationary phase from the point of 4 h; for CodYR214C and CodYR214CCcpAT19S, the exponential phase is 1–5 h, and enter the stationary phase from the time point of 5 hBack to article page