Table 1 Bacterial strains and plasmids used in this study
From: Improving l-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX
Strains or plasmids | Description | Sources |
|---|---|---|
Strains | ||
JM109 | Wild-type E. coli | NEB |
MG1655 | Wild-type E. coli K12; F− λ− rph-1 | CGSC 6300 |
TWF001 | l-Threonine-producing E. coli strain | [21] |
TSW001 | TWF001â–³proP | This study |
TSW002 | TWF001â–³proVWX | This study |
TSW003 | TWF001â–³proPâ–³proVWX | This study |
TSW004 | TWF001â–³betABIT | This study |
TSW005 | TWF001â–³proPâ–³proVWXâ–³betABIT | This study |
TSW006 | TWF001â–³proPâ–³proVWXâ–³proC | This study |
TSW007 | TWF001â–³proPâ–³proVWXâ–³fadR | This study |
TSW008 | TWF001â–³proPâ–³proVWXâ–³crr | This study |
TSW009 | TWF001â–³proPâ–³proVWXâ–³ptsG | This study |
Plasmids | ||
pCas | repA101(Ts) kan Pcas-cas9 ParaB-Red lacIq Ptrc -sgRNA-pMB1 | [27] |
pTargetF | pMB1 aadA sgRNA | [27] |
pTargetF-proP | pMB1 aadA sgRNA-proP | This study |
pTargetF-proVWX | pMB1 aadA sgRNA-proVWX | This study |
pTargetF-betABIT | pMB1 aadA sgRNA-betABIT | This study |
pTargetT-ptsG | pMB1 aadA sgRNA-ptsG | This study |
pTargetT-crr | pMB1 aadA sgRNA-crr | This study |
pTargetT-proC | pMB1 aadA sgRNA-proC | This study |