Fig. 3
From: First time β-farnesene production by the versatile bacterium Cupriavidus necator
Fed-batch fermentation process of C. necator pBBR1c_farn in a DASGIP parallel reactor system. Cultivation in minimal medium with initial volume of 300 mL at 30 °C and a final volume of 900 mL. pH regulated to 6.8 with 2 M NaOH. Aeration and stirring regulated by a regulation cascade with a DO setpoint at 30%. Induction after 9 h with 0.2% L-arabinose and addition of 100 mL n-dodecane for ISPR. Feeding start after 24.5 with varying feed rates. Performed in replicates: n = 4. Samples for β-farnesene were taken from the n-dodecane phase and related to the aqueous phase. OTR and CTR data can be found in Additional file 1