Fig. 5

Trehalose can alleviate the decrease in the oxygen consumption rate caused by heat stress. a The WT strains were transferred to new CYM medium with or without trehalose or sorbitol after 5 d of culture and treated with heat (40 °C), and b the WT strain, CK strain, and tps1 overexpression strains were treated with heat (40 °C) after culture at 28 °C for 5 d. Then, samples of the strains were collected for measurement of the oxygen respiration rate (OCR). n = 3; data are expressed as mean ± SEM values. Pro: protein. The significance analysis was conducted within CYM, Sorbitol, or Trehalose group; within WT, CK, OE::TPS1-5, or OE::TPS1-9 group separately, and the significant differences were marked with different lowercase letters over the columns (P < 0.05 according to the Tukey or Kruskal–Wallis test), the lowercase letters among different groups have no comparability in significance analysis