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Table 1 Survival of L. fermentum D12 and L. plantarum D13 after freeze-drying. (a) Influence of exopolysaccharide (EPS) addition and microencapsulation in alginate (b) Influence of the addition of lyoprotectants

From: Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain’s functionality in vitro

Lactobacillus strain

Cell survival

EPS concentration (mg/mL)

Encapsulation in alginate

0

0.2

0.5

1.0

(a)

D12

Before freeze-drying (log CFU/g)

9.77 ± 0.21az

9.99 ± 0.29az

9.79 ± 0.14az

9.62 ± 0.01az

10.13 ± 0.12az

After freeze-drying (log CFU/g)

8.19 ± 0.19bx

8.79 ± 0.17by

8.68 ± 0.09by

8.69 ± 0.09by

9.98 ± 0.04az

Cell mortality after freeze-drying (Δlog CFU/g)

1.57 ± 0.19cx

1.20 ± 0.17cxy

1.11 ± 0.09cy

0.92 ± 0.09cy

0.15 ± 0.04bz

D13*

Before freeze-drying (log CFU/g)

9.91 ± 0.01az

9.99 ± 0.04az

9.83 ± 0.06az

9.91 ± 0.07dz

10.05 ± 0.23az

After freeze-drying (log CFU/g)

9.33 ± 0.05dy

9.16 ± 0.15by

8.99 ± 0.03dy

9.12 ± 0.19by

8.39 ± 0.35cz

Cell mortality after freeze-drying (Δlog CFU/g)

0.58 ± 0.05ex

0.83 ± 0.15cy

0.84 ± 0.03ey

0.79 ± 0.19cxy

1.65 ± 0.35dz

Strain

Cell survival

Lyoprotectants (10% (w/v))

Lactose

Inulin

Sorbitol

Sucrose

(b)

D12

Before freeze-drying (log CFU/g)

10.21 ± 0.20az

10.21 ± 0.20az

10.21 ± 0.20az

10.21 ± 0.20az

After freeze-drying (log CFU/g)

10.09 ± 0.13az

9.90 ± 0.24az

10.08 ± 0.18az

9.85 ± 0.11az

Cell mortality after freeze-drying (Δlog CFU/g)

0.23 ± 0.15bz

0.30 ± 0.15bz

0.12 ± 0.15bz

0.35 ± 0.11bz

D13*

Before freeze-drying (log CFU/g)

10.18 ± 0.15az

10.18 ± 0.15az

10.18 ± 0.15az

10.18 ± 0.15az

After freeze-drying (log CFU/g)

10.08 ± 0.13ay

10.02 ± 0.18ay

9.64 ± 0.04cz

9.81 ± 0.49ay

Cell mortality after freeze-drying (Δlog CFU/g)

0.09 ± 0.11by

0.16 ± 0.17by

0.54 ± 0.04dz

0.37 ± 0.49yz

  1. abcdeDifferent letter means statistically significant difference (p < 0.05) within the same column xyzDifferent letter means statistically significant difference (p < 0.05) within the same row. The analysis was carried out using ANOVA and the results are reported as mean value ± SD of three independent experiments
  2. *L. plantarum D13 represents EPS-non producing strain