Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9

Table 1 Main process parameters obtained in the different fed-batch cultures performed for the production of active hCYP2C9/hCPR P. pastoris whole cell biocatalysts

Run	Y_X/S g_X g_S⁻¹	q_Gly g_Gly g_X⁻¹ h⁻¹	q_MetOH g_MetOH g_X⁻¹ h⁻¹	Biocatalyst activity AU L⁻¹	q_p AU g_X⁻¹ h⁻¹	Y_P/X AU g_X⁻¹	Y_P/MetOH AU g_MetOH⁻¹
FB1	0.47	0.102	–	1.78	0.67	13.4	–
FB2	0.51	0.159	–	1.52	0.78	10.4	–
FB3	0.46	0.272	–	1.17	1.71	12.9	–
FB4	0.55	0.084	0.029	3.28	1.15	21.9	39.7
FB5	0.53	0.087	0.023	2.68	1.10	21.1	47.8

µ: specific groth rate, Y_X/S: Biomass to substrate yield; q_Gly: specific glycerol uptake rate; q_MetOH: specific methanol uptake rate; q_P: specific producrtion rate; Y_P/X: Product to biomassa yield; Y_P/MetOH: Product to methanol yield;
FB1: 0.05 h⁻¹; FB2: 0.10 h⁻¹; FB3: 0.15 h⁻¹; FB4: 0.05 h⁻¹ + MetOH pulses; FB5: 0.05 h⁻¹ + MetOH constant 3 g L⁻¹

ISSN: 1475-2859