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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris

Fig. 4

The expression profiles of two gene candidates after codon optimization. a, b Top, western blot showing protein levels of PAS_chr2-2_0376 (a), PAS_chr2-2_0432 (b) before and after codon optimization; bottom, densitometric quantification of western blot results. 6 × His tag antibody and β-Actin antibody were used as primary antibodies to detect target proteins and β-actin (loading control), respectively. c qPCR showing RNA levels of PAS_chr2-2_0376 before and after codon optimization. d, e, qPCR showing RNA levels of PAS_chr2-2_0432 before and after codon optimization with 5′ primer sets (d) and 3′ primer sets (e). Error bars were calculated as standard deviation of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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