Fig. 6

Circular Dichroism (CD) spectra of 0376ori and 0376opt proteins under different temperatures. a, b Protein purification of 0376ori (a) and 0376opt (b) by affinity chromatography. In a: lane 1, flow through; lane 2, wash with 10 mM imidazole; lane 3, wash with 20 mM imidazole; lane 4, marker; lane 5, elution with 150 mM imidazole. In b: lane 1, flow through; lane 2, wash with 10 mM imidazole; lane 3, wash with 20 mM imidazole; lane 4, elution with 150 mM imidazole; lane 5, marker. Unlabeled lanes are irrelevant to this experiment. c, d CD spectra of purified 0376ori (c) and 0376opt (d) proteins under 25 °C and 95 °C. The difference in between is shadowed by grey. e Temperature mediated molecular ellipticity (208 nm) decay of 0376ori and 0376opt. Error bars were calculated as standard deviation of three independent experiments