Fig. 6
Effect of SNP treatment on ROS production in mycelium of Shiraia sp. S9. a Bright-field images (left) and fluorescence microscopy (right) of DCFH-DA-stained mycelia (400 ×). b ROS accumulation in mycelium after SNP treatment. Vc (0.01 mM) and DPI (5.00 μM) were added 30 min prior to SNP treatment. The contents of O2− c and H2O2 d in mycelium of S9 by SNP. The photos and contents of ROS were taken and detected after 2 h of SNP treatment. The SNP treatment was the same as specified in Fig. 3. The arrow represents the time of SNP addition. Values are mean ± SD from three independent experiments (*p < 0.05, **p < 0.01 vs. control and ##p < 0.01 vs. SNP)