Fig. 4

Transcriptomics and ribosome profiling reveal a translational stall site that prevents expression of P[6]. a Expression levels of the recombinant gene in strains expressing each NRRV antigen. b Gene set enrichment analysis of the P[6] strain compared to strains expressing P[4], P[8], or no recombinant protein (Null). Gene sets shown exhibit high enrichment scores and significant adjusted p-values (< 0.05) in at least one comparison. c Relative ribosome occupancy across the recombinant transcript in strains expressing P[4] or P[6]. The magnitude of occupancy is normalized to the occupancy on all transcripts in each strain. Putative stall sites represent peaks that are not reflected in the P[4] transcript, and are located near a sequence discrepancy between P[4] and P[6]. d SDS-PAGE of variants of P[6]