Fig. 1From: Promising approaches for the assembly of the catalytically active, recombinant Desulfomicrobium baculatum hydrogenase with substitutions at the active siteDifferences between the native and final, recombinant Dmb hydrogenase. (a) Schematic representation of the most prominent features of the native Dmb H2ase. Selenium in the active centre is marked in red. (b) Schematic representation of the most prominent features of the recombinant Dmb H2ase. The residues/amino acid position altered by mutagenesis (U493C) is marked in red. The N-terminal His6-tag and TEV site, resulting from the expression from pMCSG53 vector, are shown in a yellow rectangleBack to article page