Construct (sample tested) | average hydrogenase activity [µmoles H2/min/mg of protein] | standard deviation |
---|---|---|
LH U493C_STOP499_pMCSG53 + SH_pMCSG53 (in vitro assembly) | 81.368 | ± 0.15 |
SH_rbs517_LH U493C_STOP499_pMCSG53 (in vivo assembly) | 85.344 | ± 0.11 |
LH U493M_STOP499_pMCSG53 + SH_pMCSG53 (in vitro assembly) | 28.644 | ± 0.11 |
SH_rbs517_LH U493M_STOP499_pMCSG53 (in vivo assembly) | 38.278 | ± 0.01 |
LH U493M_STOP499_pMCSG53 | 0 | 0 |
LH U493C_STOP499_pMCSG53 | 0 | 0 |
SH_pMCSG53 | 0 | 0 |
assay buffer | 0 | 0 |
E. coli BL21-Gold(DE3) strain (aerobic conditions) | 0.0016 | 0 |
E. coli BL21-Gold(DE3) strain | 0.0017 | 0 |