Table 1 Strains and plasmids used in this study

Strains

BW25113

Δ(araD-araB)567ΔlacZ4787(::rrnB-3) ΔlacZ4787(::rrnB-3) Δ(rhaD-rhaB)568 hsdR514, starting strain

CGSC

E. coli DH5α

Host for plasmid construction

This study

N1

BW25113 ΔargA

This study

N2

BW25113 ΔargA ΔspeF ΔspeB

This study

N3

BW25113 ΔargA ΔspeF ΔspeB ΔastA

This study

N4

BW25113 ΔargA ΔspeF ΔspeB ΔastA ΔargR

This study

N5

BW25113 [pZE]

This study

N6

N2 [p-AGR-1]

This study

N7

N3 [p-AGR-1]

This study

N8

N4 [p-AGR-1]

This study

N9

N4 [p-AGR-2]

This study

N10

N4 [pZE]

N11

N4 [p-AGR-3, p-AGR-4]

This study

N12

N4 ΔldhA ΔadhE ΔaceE ΔpoxB ΔpflB [p-AGR-3, p-AGR-4]

This study

Plasmids

This study

p-AGR-1^a

pZE-P_LlacO1-argI-argG-argH

This study

p-AGR-2^a

pZE-P_LlacO1-carAB-argI-argG-argH

This study

p-AGR-3^a

pZE-P_LlacO1-argCBH

This study

p-AGR-4^a

pZA-P_LlacO1-argD-argG-argI

This study

pCP20

FLP recombinase expression

CGSC