A novel strategy for l-arginine production in engineered Escherichia coli

Table 2 Shake flask fermentations of strain N11 with different concentrations of yeast extract

Yeast extract (g/L)	OD₆₀₀	l-arginine (g/L)	Acetate (g/L)	∆ n-acetylglutamate (g/L)	l-arginine yields
Yeast extract (g/L)	OD₆₀₀	l-arginine (g/L)	Acetate (g/L)	∆ n-acetylglutamate (g/L)	Y_P/S^a (mol/mol)	Y_P/C^b (g/OD₆₀₀)
0	10.3 ± 0.4	2.0 ± 0.8	1.2 ± 0.1	2.4	0.9	0.19
1	13.3 ± 1	2.4 ± 0.5	6.9 ± 0.7	2.8	0.93	0.18
2	16.8 ± 0.7	4.4 ± 0.0	7 ± 1.2	4.9	0.99	0.26
4	19.5 ± 0.8	1.6 ± 0.3	3.8 ± 0.4	1.9	0.91	0.08
5	18.2 ± 1.1	1.1 ± 0.4	2.7 ± 0.4	1.3	0.92	0.06

N11: BW25113 ΔargA ΔspeF ΔspeB ΔastA ΔargR, overexpression of argCBH and argDGI
Data are indicated as means ± SD
^aY_P/S: l-arginine production yield vs. substrate (mol l-arginine/mol consumed n-acetylglutamate)
^bY_P/C: l-arginine production yield vs. biomass (g l-arginine /OD₆₀₀)

ISSN: 1475-2859