Table 3 Primers used in this study
From: A novel strategy for l-arginine production in engineered Escherichia coli
Primers | Sequence (5′ to 3′) |
|---|---|
argI-F-1 | TTAAAGAGGAGAAAGGTACCATGTCCGGGTTTTATCATAAGCATTTC |
argI-R-1 | CGTCATAGTTAATTTCTCCTACTAGTTTATTTACTGAGCGTCGCGACCAT |
argG-F-1 | CTAGTAGGAGAAATTAACTATGACGACGATTCTCAAGCATCTCCC |
argG-R-1 | GCCATAGTTAATTTCTCCTAAGCTTTTACTGGCCTTTGTTTTCCAGATTCTC |
argH-F-1 | CAGTAAAAGCTTAGGAGAAATTAACTATGGCACTTTGGGGCGGGCGTTTTAC |
argH-R-1 | TTATTTGATGCCTCTAGATTACCCTAACCGAGCCTGCGCAAAAG |
carAB-F-1 | TTCATTAAAGAGGAGAAAGGTACCATGATTAAGTCAGCGCTATT |
carAB-R-1 | TAATTTCTCCTTCTAGATTATTTGATCTGTGCGTGCATTTC |
arg-CBH-F-1 | AAGAGGAGAAAGGTACCATGTTGAATACGCTGATTGTGGGTG |
arg-CBH-R-1 | CTTTCGTTTTATTTGATGCCTCTAGATTACCCTAACCGAGCCTGCGCAAAAG |
argD-F-1 | TTCATTAAAGAGGAGAAAGGTACCATGGCAATTGAACAAACAGCAATTACA |
argD-R-1 | GTCGTCATAGTTAATTTCTCCTAAGCTTTTACGCCCCAACCACCTTCGCCACC |
argG-F-2 | AGAAATTAACTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACG |
argG-R-2 | TAGTTAATTTCTCCTACTAGTTTACTGGCCTTTGTTTTCCAGAT |
argI-F-2 | GGCCAGTAAACTAGTAGGAGAAATTAACTATGTCCGGGTTTTATCATAAGCA |
argI-R-2 | TTTGATGCCTCTAGAGCTAGCTTATTTACTGAGCGTCGCGACCATCA |
Del-argA-F | CGAATAATCATGCAAAGAGGTGTGCCATGGTGTAGGCTGGAGCTGCTTC |
Del-argA-R | GTGTTACGCATGTCGCATCCGACGATTTTCATCGCTTACCCTAAATCCGCCAT |
Del-argR-F | ATCAACCACCATATCGGGTGACTTATGGTGTAGGCTGGAGCTGCTTC |
Del-argR-R | GTCAGAAACGACGGGGCAGAGATTAAAGCTCCTGGTCGAACAGCTCT |
Del-astA-F | GCTGCTTGCGAACACTTTGTTAGCCGAGGTTCATCATGGTGTAGGCTGGAGC |
Del-astA-R | CTCTGCGCACAGGCGCACCAGACGAACATTCCGGGGATCCGTCGACC |
Del-speF-F | TGAGGACCTGCTATTACCTAAAATAAAGAGATGAAAAATGGTGTAGGCTGG |
Del-speF-R | ATTTTTCCCCTTTCAACAGGGTGCTTTGATTCCGGGGATCCGTCGACC |
Del-speB-F | TTTTTTATATCGACTTTGTAATAGGAGTCCATCCATGGTGTAGGCTGGAGCTG |
Del-speB-R | CCCTTTTTCGCCGCCTGAATATACAGATTCCGGGGATCCGTCGACC |