Fig. 5

Production of methionine by ΔmetABJ overexpressing YjeH and complemented by metY/X from CM or DG. Comparison of: (A) intracellular; (B) extracellular; and (C) total methionine levels that were quantified by GC-MS. Peak areas were normalized to ribitol internal control, and total methionine levels were calculated according to the standard methionine calibration curves. The results are presented as means ± SD of three or four replicates for each sample. Significance between bacterial strains was calculated according to the Tukey’s HSD test (p < 0.05) and is identified by different small letters. The numbers on top of the bars in each panel indicate the fold-increase relative to the WT in each panel