Fig. 1From: Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinereaConstruction and enzyme activity of T. afroharzianum engineered strains. (A) Illustration of open reading frame of chimeric protein; (B) Chitinase activities of T. afroharzianum wild-type (T30) and engineered strains. The chitinase activity of OE:SCf was higher than that of other engineered strains and wild-type (T30); Diameter of mycelium growth of T. afroharzianum engineered strains cultured in PDA medium with different inoculation methods of the colony plugs (C) and spore suspension (D); (E) Western blot analysis of chimeric protein in the culture broth of T. afroharzianum. Letters represent conditions with significant differences according to the post hoc ANOVA Fisher’s test (p < 0.05)Back to article page