Fig. 2From: Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinereaChemotropism of T. afroharzianum engineered strains. (A) Schematic representation of the plate chemotropism assay. Test compound and solvent control are applied to opposite sides of a Petri dish containing a layer of water agar with 2 × 105 /ml conidia spore of T. afroharzianum wild-type (T30), OE:Sm1, OE:Chit42, and OE:SCf at a distance of 0.5 cm from the central scoring line; (B) Directed growth of germ tubes after exposure to the indicated compounds. Letters represent conditions with significant differences according to the post hoc ANOVA Fisher’s test (p < 0.05); (C) Germination of conidia spore of T. afroharzianum over time. Germ tube emergence sites are visualized by inserted the GFP protein into T. afroharzianum wild-type (T30), OE:Sm1, OE:Chit42, and OE:SCf. DIC, differential interference contrast. Scale bar, 50 µm; (D) Mycelium chemotropism of T. afroharzianum towards cellulose (control solvent), B. cinerea cell wall, and colloidal chitin. The left filter slice is B. cinerea cell wall or colloidal chitin; The right filter slice is celluloseBack to article page