Table 1 Trials and variables levels in the Plackett–Burman experimental design and the resulted LA-EPS-20079 concentrations
Trial | pH | Incubation time (h) | Temperature (oC) | Glucose (g/l) | Peptone (g/l) | Beef extract (g/l) | Yeast extract (g/l) | K2HPO4 (g/l) | Triammonium citrate (g/l) | Sodium acetate trihydrate (g/l) | MgSO4.7H2O (g/l) | Tween-80 (ml/l) | Manganous sulfate tetrahydrate (g/l) | LA-EPS-20079 concentration (μg/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | + 1 (6.5) | + 1 (48) | + 1 (37) | + 1 (20) | + 1 (10) | + 1 (10) | + 1 (5) | + 1 (2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 417.02 |
2 | + 1 (6.5) | + 1 (48) | + 1 (37) | + 1 (20) | + 1 (10) | + 1 (10) | + 1 (5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 459.64 |
3 | + 1 (6.5) | + 1 (48) | + 1 (37) | + 1 (20) | + 1 (10) | + 1 (10) | -1 (0.5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 376.79 |
4 | + 1 (6.5) | + 1 (48) | + 1 (37) | + 1 (20) | + 1 (10) | -1 (1) | -1 (0.5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | + 1 (0.1) | 325.83 |
5 | + 1 (6.5) | + 1 (48) | + 1 (37) | + 1 (20) | -1 (1) | -1 (1) | -1 (0.5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | + 1 (1) | + 1 (0.1) | 183.21 |
6 | + 1 (6.5) | + 1 (48) | + 1 (37) | -1 (2) | -1 (1) | -1 (1) | -1 (0.5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 32.74 |
7 | + 1 (6.5) | + 1 (48) | -1 (30) | -1 (2) | -1 (1) | -1 (1) | -1 (0.5) | -1 (0.2) | -1 (0.2) | + 1 (5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 128.45 |
8 | + 1 (6.5) | -1 (24) | -1 (30) | -1 (2) | -1 (1) | -1 (1) | -1 (0.5) | -1 (0.2) | + 1 (2) | + 1 (5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 1.55 |
9 | -1 (6) | -1 (24) | -1 (30) | -1 (2) | -1 (1) | -1 (1) | -1 (0.5) | + 1 (2) | + 1 (2) | + 1 (5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 3.45 |
10 | -1 (6) | -1 (24) | -1 (30) | -1 (2) | -1 (1) | -1 (1) | + 1 (5) | + 1 (2) | + 1 (2) | + 1 (5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 112.97 |
11 | -1 (6) | -1 (24) | -1 (30) | -1 (2) | -1 (1) | + 1 (10) | + 1 (5) | + 1 (2) | + 1 (2) | + 1 (5) | + 1 (2) | + 1 (1) | + 1 (0.1) | 137.27 |
12 | -1 (6) | -1 (24) | -1 (30) | -1 (2) | + 1 (10) | + 1 (10) | + 1 (5) | + 1 (2) | + 1 (2) | + 1 (5) | + 1 (2) | + 1 (1) | -1 (0.05) | 13.45 |
13 | -1 (6) | -1 (24) | -1 (30) | + 1 (20) | + 1 (10) | + 1 (10) | + 1 (5) | + 1 (2) | + 1 (2) | + 1 (5) | + 1 (2) | -1 (0.1) | -1 (0.05) | 419.88 |
14 | -1 (6) | -1 (24) | + 1 (37) | + 1 (20) | + 1 (10) | + 1 (10) | + 1 (5) | + 1 (2) | + 1 (2) | + 1 (5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 399.17 |
15 | -1 (6) | + 1 (48) | + 1 (37) | + 1 (20) | + 1 (10) | + 1 (10) | + 1 (5) | + 1 (2) | + 1 (2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 379.17 |
16 | -1 (6) | -1 (24) | -1 (30) | -1 (2) | -1 (1) | -1 (1) | -1 (0.5) | -1 (0.2) | -1 (0.2) | -1 (0.5) | -1 (0.2) | -1 (0.1) | -1 (0.05) | 239.41 |