Table 2 Confirmation trials of Plackett–Burman design

PH

6

6

6

6

Incubation time (h)

48

48

48

48

Temperature (ºC)

30

30

30

30

Glucose (g/l)

20

20

20

20

Peptone (g/l)

1

-------

1

1

Beef extract (g/l)

10

10

10

10

Yeast extract (g/l)

5

5

5

5

K₂HPO₄ (g/l)

0.2

-------

0.2

0.2

Triammonium citrate (g/l)

0.2

-------

0.2

0.2

Sodium acetate trihydrate (g/l)

5

5

5

5

MgSO₄.7H₂O (g/l)

2

2

2

2

Tween-80 (ml/l)

0.1

-------

-------

-------

Manganous sulfate tetrahydrate (g/l)

0.1

0.1

0.1

-------