Table 3 Box–Behnken factorial design demonstrating LA-EPS-20079 concentration as a response to changing glucose, yeast extract, and sodium acetate trihydrate levels
Trial | Glucose (g/l) | Yeast extract (g/l) | Sodium acetate trihydrate (g/l) | Oligosaccharides concentration (μg/ml) | ||
|---|---|---|---|---|---|---|
Measured | Predicted | |||||
1 | -1 (20) | -1 (5) | 0 (7.5) | 485 | 480.65 | |
2 | + 1 (30) | -1 (5) | 0 (7.5) | 543.095 | 583.75 | |
3 | -1 (20) | + 1 (10) | 0 (7.5) | 442.62 | 401.96 | |
4 | + 1 (30) | + 1 (10) | 0 (7.5) | 675 | 679.3 | |
5 | -1 (20) | 0 (7.5) | -1 (5) | 423.57 | 454.70 | |
6 | + 1 (30) | 0 (7.5) | -1 (5) | 516.90 | 503.045 | |
7 | -1 (20) | 0 (7.5) | + 1 (10) | 417.86 | 431.73 | |
8 | + 1 (30) | 0 (7.5) | + 1 (10) | 794 | 763.87 | |
9 | 0 (25) | -1 (5) | -1 (5) | 458.33 | 431.55 | |
10 | 0 (25) | + 1 (10) | -1 (5) | 571.19 | 580.714 | |
11 | 0 (25) | -1 (5) | + 1 (10) | 700.71 | 691.19 | |
12 | 0 (25) | + 1 (10) | + 1 (10) | 532.14 | 558.93 | |
13 | 0 (25) | 0 (7.5) | 0 (7.5) | 545.95 | 546.90 | |
14 | 0 (25) | 0 (7.5) | 0 (7.5) | 547.86 | 546.90 | |