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Table 3 Matrix of Plackett–Burman experimental design

From: Production, bioprocessing and antiproliferative activity of camptothecin from Aspergillus terreus, endophyte of Cinnamomum camphora: restoring their biosynthesis by indigenous microbiome of C. camphora

Std. order

X1

X2

X3

X4

X5

X6

X7

X8

X9

X10

X11

X12

X13

X14

X15

X16

X17

X18

X19

CPT yield (µg/l)

1

7

7

0.5

2

0.1

4

10

4

10

2

0.4

8

0.1

1

4

0.4

1

15

0.1

22.4

2

7

16

0.5

2

0.1

4

4

10

4

4

0.1

8

0.4

1

1

0.4

4

2

0.4

96.8

3

7

16

0.5

10

0.4

4

4

4

10

2

0.1

2

0.4

4

1

0.1

4

15

0.1

87.2

4

7

16

10

10

0.1

4

4

4

4

4

0.4

2

0.1

4

4

0.1

1

15

0.4

127.6

5

7

16

10

10

0.4

8

4

4

4

2

0.1

8

0.1

1

4

0.4

1

2

0.4

72.4

6

2

16

10

10

0.1

8

10

4

4

2

0.4

2

0.4

1

1

0.4

4

2

0.1

45.6

7

7

7

10

10

0.1

8

10

10

4

2

0.1

8

0.1

4

1

0.1

4

15

0.1

90

8

2

16

10

2

0.1

8

10

10

10

2

0.1

2

0.4

1

4

0.1

1

15

0.4

25.2

9

7

7

0.5

10

0.1

8

10

10

10

4

0.1

2

0.1

4

1

0.4

1

2

0.4

73.6

10

7

16

10

2

0.4

4

10

10

10

4

0.1

2

0.1

1

4

0.1

4

2

0.1

87.2

11

2

16

0.5

10

0.4

8

4

10

10

4

0.4

2

0.1

1

1

0.4

1

15

0.1

36.4

12

2

7

10

10

0.4

4

10

4

10

4

0.4

8

0.1

1

1

0.1

4

2

0.4

44.8

13

7

7

10

2

0.4

8

4

10

4

4

0.4

8

0.4

1

1

0.1

1

15

0.1

85.6

14

7

16

0.5

2

0.4

8

10

4

10

2

0.4

8

0.4

4

1

0.1

1

2

0.4

45.6

15

2

16

0.5

10

0.1

4

10

10

4

4

0.4

8

0.4

4

4

0.1

1

2

0.1

36.4

16

2

7

10

10

0.4

4

4

10

10

2

0.1

8

0.4

4

4

0.4

1

2

0.1

20.4

17

7

7

10

2

0.1

8

4

4

10

4

0.4

2

0.4

4

4

0.4

4

2

0.1

90

18

2

16

0.5

2

0.4

8

10

4

4

4

0.1

8

0.1

4

4

0.4

4

15

0.1

36.4

19

7

7

0.5

10

0.4

4

10

10

4

2

0.4

2

0.4

1

4

0.4

4

15

0.4

45.6

20

2

16

10

2

0.1

4

4

10

10

2

0.4

8

0.1

4

1

0.4

4

15

0.4

44.8

21

2

7

0.5

10

0.1

8

4

4

10

4

0.1

8

0.4

1

4

0.1

4

15

0.4

44.8

22

2

7

10

2

0.4

4

10

4

4

4

0.1

2

0.4

4

1

0.4

1

15

0.4

16.8

23

2

7

0.5

2

0.4

8

4

10

4

2

0.4

2

0.1

4

4

0.1

4

2

0.4

22.4

24

2

7

0.5

2

0.1

4

4

4

4

2

0.1

2

0.1

1

1

0.1

1

2

0.1

20.4

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Microbial Cell Factories

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