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Table 6 Matrix and responses of the CCD for the significant physical and chemical factors affecting on the production of CPT

From: Production, bioprocessing and antiproliferative activity of camptothecin from Aspergillus terreus, endophyte of Cinnamomum camphora: restoring their biosynthesis by indigenous microbiome of C. camphora

Std. order

X2

XI

X4

X3

X16

X5

CPT yield (μg/l)

1

7

3

5

5

0.4

0.4

2.40

2

22

3

5

5

0.4

0.8

0.06

3

7

11

5

5

0.4

0.8

61.20

4

22

11

5

5

0.4

0.4

156.00

5

7

3

20

5

0.4

0.8

39.00

6

22

3

20

5

0.4

0.4

72.60

7

7

11

20

5

0.4

0.4

79.20

8

22

11

20

5

0.4

0.8

120.60

9

7

3

5

20

0.4

0.8

61.20

10

22

3

5

20

0.4

0.4

61.20

11

7

11

5

20

0.4

0.4

108.00

12

22

11

5

20

0.4

0.8

120.60

13

7

3

20

20

0.4

0.4

1.20

14

22

3

20

20

0.4

0.8

4.80

15

7

11

20

20

0.4

0.8

97.20

16

22

11

20

20

0.4

0.4

145.20

17

7

3

5

5

0.8

0.8

7.20

18

22

3

5

5

0.8

0.4

9.00

19

7

11

5

5

0.8

0.4

90.60

20

22

11

5

5

0.8

0.8

187.20

21

7

3

20

5

0.8

0.4

4.20

22

22

3

20

5

0.8

0.8

75.00

23

7

11

20

5

0.8

0.8

99.00

24

22

11

20

5

0.8

0.4

181.20

25

7

3

5

20

0.8

0.4

7.20

26

22

3

5

20

0.8

0.8

3.60

27

7

11

5

20

0.8

0.8

8.40

28

22

11

5

20

0.8

0.4

74.40

29

7

3

20

20

0.8

0.8

54.00

30

22

3

20

20

0.8

0.4

60.60

31

7

11

20

20

0.8

0.4

61.26

32

22

11

20

20

0.8

0.8

157.80

33

3.4

7

12.5

12.5

0.6

0.6

60.00

34

32.4

7

12.5

12.5

0.6

0.6

120.60

35

14.5

2.6

12.5

12.5

0.6

0.6

30.00

36

14.5

16.6

12.5

12.5

0.6

0.6

75.00

37

14.5

7

5.34

12.5

0.6

0.6

72.60

38

14.5

7

30.4

12.5

0.6

0.6

99.00

39

14.5

7

12.5

5.4

0.6

0.6

120.60

40

14.5

7

12.5

30.4

0.6

0.6

159.00

41

14.5

7

12.5

12.5

0.3

0.6

132.60

42

14.5

7

12.5

12.5

1.1

0.6

60.00

43

14.5

7

12.5

12.5

0.6

0.2

91.20

44

14.5

7

12.5

12.5

0.6

1.1

75.00

45

14.5

7

12.5

12.5

0.6

0.6

90.60

46

14.5

7

12.5

12.5

0.6

0.6

117.00

47

14.5

7

12.5

12.5

0.6

0.6

120.00

48

14.5

7

12.5

12.5

0.6

0.6

120.00

49

14.5

7

12.5

12.5

0.6

0.6

157.20

50

14.5

7

12.5

12.5

0.6

0.6

111.00

51

14.5

7

12.5

12.5

0.6

0.6

118.80

52

14.5

7

12.5

12.5

0.6

0.6

145.20

53

14.5

7

12.5

12.5

0.6

0.6

181.20