Fig. 1

Overview of vital carbon metabolism pathways (glucose metabolism, xylose metabolism, exopolysaccharide synthesis, and PHB synthesis) in NX02. Arrows indicate the pathway steps encoded by the adjacent labeled gene products. Black arrows indicate the native pathways. Green arrows indicate the overexpressed pathways by addition of copies of native enzymes. Dotted black arrows indicate pathways abolished by deletion of the first glycosyltransferase (ssB). The white oval granules represent the product PHB. Orf 00050, orf 02310, orf 02740, orf 06335, orf 09175, orf 10285, orf 13370, orf 14780, and orf 14815 represent genes encoding isoenzyme of β-ketothiolase; Orf 02315, orf 02745, orf 02775, orf 02830, orf 09170, orf 09185, and orf 26300 represent genes encoding isoenzyme of acetoacetyl-CoA reductase; Orf 00055, orf 02300, orf 02730, orf 09265, orf 09550, orf 20850, orf 22525, and orf 28645 represent genes encoding isoenzyme of PHB synthase. Abbreviations: EMP, Embden-Meyerhof-Parnas; ED, Entner-Doudoroff; PP, pentose phosphate; TCA, tricarboxylic acid; phbA, encoding β-ketothiolase; phbB, encoding NADPH-dependent acetoacetyl-CoA reductase; phbC, encoding PHB synthase; xylB, encoding xylose dehydrogenase; xylC, encoding xylonolactonase; kdpgA, encoding 2-keto-3-deoxy D-xylonate aldolase