Table 2 All primers used in this study
Primer name | Primer sequence (5→3) |
|---|---|
Primers for genetic manipulation | |
pBTBC_F | GAAAGGGACGAGGATGGCGAGGAGCTCCAATTCGCCCTAT |
pBTBC_R | GATCCCCAGCGACCAGGCGGCCCGGTACCTTCTCCTTA |
pBTBCA_F | GTTTCGGGATTGAGGACCAGGGGAGCTCCAATTCGCCCTAT |
BC_F | TAAGGAGAAGGTACCGGGCCGCCTGGTCGCTGGGGATC |
BC_R | ATAGGGCGAATTGGAGCTCCTCGCCATCCTCGTCCCTTTC |
BCA_R | CTTTCCCGGTGTCTCGTTGTGTCGCCATCCTCGTCCCTTTC |
kdpgA_F | GAAAGGGACGAGGATGGCGACACAACGAGACACCGGGAAAG |
kdpgA_R | ATAGGGCGAATTGGAGCTCCCCTGGTCCTCAATCCCGAAAC |
Primers for RT-PCR | |
RT16S_F | TCAAACGCTGGTAAGGTTCTGC |
RT16S_R | GCGGCTCACTGGACTGGTATT |
RTssB_F | CGTTCAGGATCTGCGGCAG |
RTssB_R | ATCTGGCCCTGACGCTTGC |
RTxylB_F | TGAATGCGTCGATCGAGGTT |
RTxylB_R | GTAGAGGACGAGCTCGGGCA |
RTxylC_F | TGTGGTTCGGCAGCATGGAC |
RTxylC_R | GATATGCTTCACCGGCGTGC |
RTkdpgA_F | CTTCACCCACCTCTCCGCC |
RTkdpgA_R | GATCATCGCGGTCTGGGTG |