Fig. 1From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coliOverview of lanthipeptide expression strains constructed in this study. A The NisSTldhT7 mCherry-NisA heterologous expression strain was co-transformed with plasmids pRSTcnA*nB and pACYCNisCi. B Strain NisSTldhSTldh was co-transformed with pRSScnA*nB and pACYCNisCi. The Pre-ClausA and Pre-EpiA-producing strains were co-transformed with pRSTccA*cB (for clausin) or pRSTceA*cB (for epidermin) and plasmids pClausCi and pClausDi to yield strains C ClausSTldhT7 and EpiSTldhT7, respectively. Relevant promoter and gene combinations are indicated in D. Figure created in biorender (http://biorender.io)Back to article page