Fig. 7From: Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coliAntimicrobial activity and optimization results for mCherry-PreNisA expressed in M9 minimal media, LB, BHI, TBG (TB containing 0.5% glycerol) and TB-G (TB without 0.5% glycerol). Antimicrobial activity of IMAC-purified mCherry-PreNisA produced by cultures that were A induced (On) with 0.15Â mM IPTG and 0.5% glycerol (v/v) and those that were kept B un-induced (off). Uncleaved (UC) and hNisP cleaved (C) activity results are shown for all samples tested. Antimicrobial activity was assessed using an agar well diffusion assay against L. sakeiBack to article page