Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase

Table 1 The physicochemical properties and substrate specificity of the characterized R-BDH

Strains	Substrate	Product	K_m	Optimal pH	Optimal temperatures	Reference
Bacillus sp. DL01	DA	3R-AC	0.626 ± 0.12	7.4	40	(1)
	3R/3S-AC	(2R,3R)/meso-2,3-BD	0.253 ± 0.003	5.5	35
	meso-2,3-BD	3S-AC	0.397 ± 0.11	7.4 ~ 8	40
	(2R,3R)-2,3-BD	3R-AC	0.389 ± 0.090	7.4 ~ 8	40
R. erythropolis WZ010	DA	3R-AC	0.1 ± 0.001	ND	55	(2)
	3R/3S-AC	(2R,3R)/meso-2,3-BD	ND	6.5	55
	meso-2,3-BD	3S-AC	ND	10	45
	(2R,3R)-2,3-BD	3R-AC	0.58 ± 0.05	10	45
P. polymyxa ZJ-9	3R-AC	(2R,3R)-2,3-BD	0.20 ± 0.02	6.0	30	(3)
	3S-AC	meso-2,3-BD	0.84 ± 0.05	6.0
	meso-2,3-BD	3S-AC	2.73 ± 0.02	8.0
	(2R,3R)-2,3-BD	3R-AC	7.67 ± 0.73	8.0
B. thuringiensis	DA	3R-AC	0.66 ± 0.06	7.5	35	(4)
	3R/3S-AC	(2R,3R)/meso-2,3-BD	0.49 ± 0.08	7.5	35
	meso-2,3-BD	3S-AC	0.76 ± 0.12	10	50
	(2R,3R)-2,3-BD	3R-AC	3.67 ± 0.55	10	50

ISSN: 1475-2859