Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase

Table 2 The physicochemical properties and substrate specificity of the characterized R-BDH

Strains	Substrate	Product	K_m	Optimal pH	Optimal temperatures	Reference
K. pneumoniae XJ-Li	DA	3S-AC	ND	ND	ND	(5)
	3R/3S-AC	meso/(2 S,3 S)-2,3-BD	0.65 ± 0.11	8.0	35
	meso-2,3-BD	3R-AC	13 ± 0.32	9.0
	(2S,3S)-2,3-BD	3S-AC	ND	9.0
S. marcescens H30	DA	3S-AC	3.3 ± 0.1	8.0	ND	(6)
	3R/3S-AC	meso/(2 S,3 S)-2,3-BD	0.97 ± 0.08	5.0	40
	meso-2,3-BD	3R-AC	4.1 ± 0.3	8.0
	(2S,3S)-2,3-BD	3S-AC	31.2 ± 0.4	ND
B. licheniformis	DA	3S-AC	72.4 ± 0.4	5.0	ND	(7)
	3R/3S-AC	meso/(2S,3S)-2,3-BD	0.47 ± 0.03	5.0	37
	meso-2,3-BD	3R-AC	7.25 ± 0.05	10.0
	(2S,3S)-2,3-BD	3S-AC	7.25 ± 0.05	10.0

ISSN: 1475-2859