Fig. 4From: Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3The alginate lyase production of strain A3 cultured under the conditions before and after optimization. Before optimization, strain A3 was cultured in the medium (pH 8.0) containing 1% (w/v) L. japonica root powder and 3% sea salts with 1% inoculum size at 20 °C, 180 rpm for 24 h. After optimization, strain A3 was cultured in the medium (pH 8.04) containing 2.03% NH4NO3, 0.6% L. japonica root powder and 3% sea salt with 2% inoculum size at 19.6oC, 120 rpm for 36 h. The graphs show data from triplicate experiments (mean ± S.D.)Back to article page