Fig. 7From: Extracellular vesicles produced by avian pathogenic Escherichia coli (APEC) activate macrophage proinflammatory response and neutrophil extracellular trap (NET) formation through TLR4 signalingInhibition of TLR4 suppressed NET formation in neutrophils. (A) CT265 EVs (50 µg/mL) were incubated with TAK242-pretreated mouse neutrophils for 120 min. Fluorescence microscopy showed that the neutrophils did not release NETs. (B) CT265 EVs (50 µg/mL) were incubated with TLR4 knockout (KO) mice neutrophils for 120 min. Fluorescence microscopy showed that the neutrophils did not release NETs. Neutrophils from TLR4 KO mice did not support NET formationBack to article page