Table 1 Plasmids and strains used in this work (Ap^R: ampicillin-resistance; Tsr^R: thioestrepton-resistance; Am^R: apramycin-resistance)

pSEVAUO-M21703

φBT1, Ap^R-Tsr^R. Level 2 MoClo receptor vector for naringenin BGC cloning

(9)

pSEVAUO-M21102-TAL

φBT1, Ap^R. Level 1 MoClo donor plasmid containing P_ermE*-TAL

(9)

pSEVAUO-M21202-4CL

φBT1, Am^R. Level 1 MoClo donor plasmid containing SF14-4CL

(9)

pSEVAUO-M21302-CHS

φBT1, Am^R. Level 1 MoClo donor plasmid containing SP25-CHS

(9)

pSEVAUO-M21402-CHI

φBT1, Am^R. Level 1 MoClo donor plasmid containing SP43-CHI

(9)

pSEVAUO-M21703-NarBGC

φBT1, Ap^R-Tsr^R. It harbors the naringenin BGC

This work

Strain

Use in this work

Reference or source

Escherichia coli Top10

Routine sub-cloning and DNA propagation

Invitrogen

S. albidoflavus WT-NAR

S. albidoflavus J1074 with naringenin BGC integrated into φC31 site

(9)

S. albidoflavus UO-FLAV-002-NAR

S. albidoflavus UO-FLAV-002 with naringenin BGC integrated into φC31 site

(9)

S. albidoflavus WT-NARNAR

S. albidoflavus J1074 with two copies of naringenin BGC, one integrated into φC31 site and another integrated into φBT1 site

This work

S. albidoflavus UO-FLAV-002-NARNAR

S. albidoflavus UO-FLAV-002 with two copies of naringenin BGC, one integrated into φC31 site and another integrated into φBT1 site

This work