Table 4 Mutations reported for deregulation of feedback inhibition by amino acids against enzyme targets
From: Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method
Enzyme | Mutants | Deregulation of Feedback Inhibition | Reference |
|---|---|---|---|
NAGK (C. glutamicum) | H271N and E284D E19R, H26E, R209A, H268N, G287D | Markedly enhanced Arginine production Moderate effect on inhibition tendency of Arginine | [189] |
NAGK (C. crenatum) | E19F, E19L, E19A E19G, E19R | Restrict binding of arginine Enhanced arginine tendency | [190] |
HisG (C. glutamicum) | N215K/L231F/T235A G233H/T235Q S143F | Increased Histidine production Strongly deregulated Histidine Feedback inhibition | |
DHDPS (C. glutamicum) | H56K, E84T A49P, A49W, L51T | Total deregulation of Histidine feedback inhibition Slight deregulation of Histidine Feedback inhibition | [97] |
DHDPS (E. coli) | R138H, R138A | – | [68] |
DAPDC | Q381A, R385A | Negative effect on catalytic activity | [191] |
Aspartokinase III (E. coli) | T352I, S300T S369F, E164K T344M, S345L, G323D | Total deregulation of feedback inhibition No effect of feedback inhibition Considerable lysine insensitivity | |
Aspartokinase (C. glutamicum) | Q298G, I272E, N372A, I375P Glu382Ala T361A, S381F, A279T | Complete deregulation of Lysine feedback inhibition Moderate deregulation of Lysine feedback inhibition No effect on deregulation Highest resistance towards feedback inhibition of Lysine | |
AKI-HDI (S. marcescens) | S352F | Highest resistance towards feedback inhibition | [193] |
HSK (C. glutamicum) | A20G | Significantly reduces threonine feedback inhibition | [194] |
HSK (E. coli) | H202L, R234L, R234C, R234H, H139L | Marked effect on catalytic activity | [195] |
SAT (E. histolytica) | H208S | Marked decrease in cysteine sensitivity | [196] |
SAT (E. coli) | M256I, M201V, E166G | Significant insensitivity towards Cysteine inhibition | |
α-IPMS (M. tuberculosis) | R80A, R80K, K79A, N83A Y410F, E218A | Destroyed enzymatic activity Slow onset of feedback inhibition Complete loss of leucine sensitivity | |
Chorismate mutase (S. cerevisiae) | E246Q N194D | Profoundly affect catalytic activity No effect on catalytic and regulatory properties | |
Chorismate mutase (E. coli) | A354V and F357L | Tyrosine resistant | [202] |