Fig. 4

Verification of evolved strain C. glutamicum WT_EtOH-Evo. (A) Experimental design for automated microscale experiments using one FlowerPlate and defined CGXII medium with either 55 mM glucose or 428 mM ethanol as sole carbon and energy source, respectively. In triplicates, repetitive cultures were performed on glucose. After each glucose culture, a characterization on ethanol was performed. (B) Growth performances of C. glutamicum WT_EtOH-Evo in the 24 batch cultures on ethanol. Mean values and standard deviations were estimated from each of the three parallel cultures and are shown as lines and shaded areas, respectively. (C) Estimated specific growth rates across the batch cultures on ethanol